Liver fibrosis is a reversible pathology characterized by the up-regulated secretion and deposition of ECM proteins and inhibitors of metalloproteinases, which increase the stiffness and viscosity of this organ. Since recent studies have shown that fibrosis preceded the generation of hepatocellular carcinomas, we hypothesize that liver fibrosis could play a role as a mechanism for…
En este trabajo se presentan resultados de la síntesis de hidroxiapatita (HA) en sílice mesoporosa SBA-15. Se ha hecho un estudio de la síntesis de ambos materiales y un seguimiento del efecto del doble tratamiento térmico posterior a la síntesis. Las muestras se sometieron a distintas temperaturas de tratamiento hidrotermal entre 353 y 393 K…
Analyses of the pore size distribution in 3D matrices such as the cell-hydrogel interface are very useful when studying changes and modifications produced as a result of cellular growth and proliferation within the matrix, as pore size distribution plays an important role in the signaling and microenvironment stimuli imparted to the cells. However, the majority…
Primary mouse hepatocytes (between 10-250 cells per capsule) were immobilized within 1.0% w/v alginate microbeads. The textural properties of the alginate matrix were characterized and a full protocol based upon the measurement of the initial rate of Resazurin reduction was studied and standardized. Using this method, the decay rate constant (K-d = 0.45 +/- 0.01…