NiSn bimetallic catalysts supported over Al2O3 modified with different promoter (Mg and/or Ce) were prepared and characterized by powder X-ray diffraction (XRD), N2 sorptometry, and temperature programmed reduction (TPR). Hydrogen production by glycerol steam reforming over these catalysts was investigated. Among the catalysts, NiSn/AlMgCe catalyst shows the highest hydrogen yield as well as the best stability during the reaction. The effect of reaction temperature, water/glycerol molar ratio and space velocity on the glycerol steam reforming over NiSn/AlMgCe were also investigated. Finally, it was verified that the catalyst can be regenerated by oxidation of carbonaceous deposits.